BIO-ONE
The Bio-One franchise provides restoration services focused on the residential and commercial removal of regulated and non-regulated bio-medical waste. It offers additional services such as cleaning, disinfecting, hoarding remediation, medical waste disposal, and sharp-instruments removal. The business model involves franchising these specialized biohazard cleanup services to operators targeting property owners, businesses, and individuals needing professional remediation.
Franchise Costs
7.5% royalty + 2% marketing fund + $650/mo technology maintenance fee (+ $0-250/mo accounting software)
Financial Performance
Item 19 Financial Performance
Data Based On: 113 franchised outlets open for at least 12 months in 2024 for revenue data; 79 of these provided P&L reports for expense and EBITDA data.
* Average gross revenue was calculated as a weighted average of the average revenues for 4 quartiles based on their respective unit counts: (28 units × $762,596 + 28 units × $441,299 + 28 units × $248,139 + 29 units × $113,506) / 113 total units = $388,925.89. Revenue top quartile is the average revenue of Quartile 1 ($762,596). Revenue bottom quartile is the average revenue of Quartile 4 ($113,506). Median gross revenue was not explicitly provided as an overall system-wide median. Average EBITDA was calculated by multiplying the weighted average gross revenue ($388,925.89) by the average Adjusted EBITDA margin (18.69%) provided in the '2024 Average Expenses as Percentage of Average Revenue' table. The best guess profit range was estimated using the average and median Adjusted EBITDA margins from the '2024 Average Expenses as Percentage of Average Revenue' table. Best guess profit low uses the average EBITDA margin (18.69% × $388,925.89 = $72,690.25). Best guess profit high uses the median EBITDA margin (24.26% × $388,925.89 = $94,353.42). These are EBITDA-based estimates, not net profit or net income. Revenue data is based on 113 franchisees, while expense and EBITDA data are based on 79 franchisees who submitted P&L reports.
Extracted Item 19 Section
How BIO-ONE Compares
Key Insights
- Lower than average SBA loan default rate in Home Services
| Franchise | Investment | Fee | Royalty | Locations |
|---|---|---|---|---|
BIO-ONE Current | $132K – $218K | $60K | 7.5% | 137 |
| MERRY MAIDS | $127K – $166K | $55K | 7.0% | 933 |
| HOLIDAY LIGHTING HEROES | $150K – $177K | $127K | 10.0% | 653 |
| SIGN GYPSIES | $4K – $5K | $1K | N/A | 587 |
| MOSQUITO AUTHORITY | $54K – $128K | $45K | 10.0% | 546 |
| Culligan | $130K – $814K | $40K | 2.0% | 460 |
Home Services Average 130 franchises | $144K – $289K | $53K | 7.0% | – |
* Comparison based on latest FDD filings. Investment ranges from Item 7, fees from Item 5. Showing top 5 of 130 Home Services franchises by location count.
Locations & Growth
Outlet Growth Over Time
Total outlets at end of each year
Geographic Distribution (2024)
Outlets by state across the United States
+31 more states
SBA Loan History
Historical SBA 7(a) loan data for BIO-ONE franchisees (2016 – 2025)
Loans by Year
SBA 7(a) loan activity over time
* Data sourced from SBA 7(a) FOIA loan records. Default rate calculated from charged-off loans.
Franchisee Contacts
15 franchisee contacts on file from official FDD filings.
| Name | SBA Loan |
|---|---|
John Smith ABC Franchise LLC | $350,000 2023 · Active |
John Smith ABC Franchise LLC | $350,000 2023 · Active |
John Smith ABC Franchise LLC | $350,000 2023 · Active |
John Smith ABC Franchise LLC | $350,000 2023 · Active |
John Smith ABC Franchise LLC | $350,000 2023 · Active |
15 Contacts Available
Unlock all BIO-ONE franchisee contacts with verified contact information
Due Diligence
Litigation (Item 3)
Breach of contract and required supplies disputes with a former franchisee.
Bankruptcy (Item 4)
System Health (Item 20)
Franchise system changes reported in the most recent fiscal year
Frequently Asked Questions
The total initial investment to open a BIO-ONE franchise ranges from $132,145 to $217,595. This includes a franchise fee of $60,000. Ongoing royalty fees are 7.5% of gross sales.
Unlock Full FDD Access
Download BIO-ONE's complete FDD plus 1,700+ other franchise documents — all with one plan.
Secure checkout with Stripe · Cancel anytime
Also includes FDDs for
More Services Franchises
THE UPS STORE (TRADITIONAL)
Services
AMERIPRISE FINANCIAL SERVICES LLC
Services
JACKSON HEWITT TAX SERVICE
Services
SERVPRO (UNIT)
Services
H&R BLOCK
Services
SERVICEMASTER RESTORE
Services
